TABLE 2.
Genes mutated to investigate the biosynthesis of the cryptic LPS glycoforms in H. influenzae
| Genea | Function | Reference | Effect of mutation on cryptic glycoforms of strainb:
|
|
|---|---|---|---|---|
| RM118 | RM153 | |||
| cld (HI0866) | 13 | (+) | − | |
| lsg1 (HI0867) | 13 | − | − | |
| kfiC (HI0868) | 13 | + | + | |
| orfE (HI0869) | 13 | + | + | |
| orfO (HI0870) | 13 | − | − | |
| orfY (HI0871) | 13 | (+)c | (+) | |
| wbaP (HI0872) | 13 | + | + | |
| rmlB (HI0873) | 13 | − | − | |
| cap5j (HI0874) | + | + | ||
| lsgA (HI1700) | 1 | + | (+)c | |
| lsgB (HI1699) | 1 | (+)c | + | |
| lsgC (HI1698) | 1 | (+)c | NDd | |
| lsgD (HI1697) | 1 | − | − | |
| lsgE (HI1696) | 1 | − | − | |
| lsgF (HI1695) | 1 | − | − | |
| orfM (HI0260 | 13 | − | − | |
| mrsA (HI1337) | 13 | − | − | |
| wecA (HI1716) | 13 | (+)c | (+) | |
| lgtF (HI0653) | β-Glucosyltransferase | 9 | +c | +c |
| lgtC (HI0258) | α-Galactosyltransferase | 13 | −c | − |
| lpsA (HI0765) | β-Glycosyltransferase | 9,13 | −c | −c |
| lex2B | β-Glucosyltransferase | 14 | ND | − |
| pgmB (HI0740) | Phosphoglucomutase | 13 | +a | +c |
| lic2C | ND | −c | ||
| siaB (HI1279) | CMP-Neu5Ac synthetase | 12 | + | + |
| lic1 (HI1537-40) | Addition of phosphocholine | 34 | − | − |
| lic2A (HI0550) | β-Galactosyltransferase | 9 | − | − |
| kdkA (HI0260.1) | KDO kinase | 13,36 | −c | − |
a
The genes in the lower part of the table are those which have a characterized function in H. influenzae LPS synthesis.
b
LPS profiles for a majority of mutant strains are represented in Fig. 2. +, (+), and −, loss of, reduction in intensity of, and no effect on the cryptic LPS glycoforms observed compared to wild type.
c
LPS profile indicates that mutation has other effects upon core LPS glycoforms.
d
ND, not determined.