FIG. 3.

Protein compositions of S. pyogenes wild-type and rgg mutant strains after 24 h. Proteins were isolated from wild-type and rgg mutant strains after 24 h of culture, separated by 2-DE, and stained with Sypro Ruby. The migration positions of molecular weight standards and the orientation of the immobilized pH gradients are indicated. The numbers on the gel correspond to the SPy numbers in the serotype M1 genome sequence annotation (14) and designate proteins identified by tandem mass spectrometry. The original gel size was 25 by 21 by 0.1 cm.