TABLE 1.
Number of protein differences between NZ131 and NZ131 rgg− during growtha
| Parameter | No. of spots after:
|
||
|---|---|---|---|
| 4 h | 24 h | 48 h | |
| Interstrain differences in protein spot abundanceb | 38 | 27 | 41 |
| Present only in wild-type samples | 20 | 17 | 22 |
| Present only in rgg mutant samples | 30 | 33 | 36 |
a
Proteins were isolated from NZ131 and NZ131 rgg− after 4, 24, and 48 h of culture and separated by 2-DE. The results from at least two independent experiments were analyzed with the PDQuest software to identify differences in protein composition and protein abundance between the strains. Protein spots were quantified by adding the pixel values for a spot.
b
Differences in protein abundance between the isogenic strains at P < 0.05.