TABLE 2.
Migration and abundance of selected proteins associated with amino acid metabolism isolated from wild-type and rgg mutant strains
| SPy no.a | Gene product | Observed pI/Mr | Predicted pI/Mr | Mean amt (SEM) after growth forb:
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| 4 h
|
24 h
|
48 h
|
|||||||
| Wild type | rgg mutant | Wild type | rgg mutant | Wild type | rgg mutant | ||||
| 1541 | ArcC | 4.7/32.7 | 4.8/33.1 | NDc | 5,434 (170) | 1,786 (397) | 8,673 (381) | 2,624 (1,176) | 3,934 (1,134) |
| 1541 | ArcC | 4.6/33.0 | 4.8/33.1 | ND | 1,048 (1,048) | 199 (199) | 763 (763) | ND | 339 (90) |
| 1544 | ArcB | 5.4/37.9 | 5.3/37.9 | ND | 5,202 (803) | ND | 2,373 (1,114) | 644 (302) | 1,485 (228) |
| 1547 | ArcA/SagP | 5.1/47.7 | 5.0/46.3 | ND | 4,425 (381) | 1,338 (1,338) | 3,606 (299) | 2,173 (539) | 9,542 (6,031) |
| 2081 | HutI | 5.6/41.7 | 5.5/46.8 | ND | 2,726 (2,726) | 779 (482) | 1,769 (1,769) | ND | 4,355 (1,799) |
| 2190 | SdhA | 4.8/27.7 | 4.7/30.0 | ND | 3,073 (83) | ND | 685 (685) | 2,795 (496) | 2,595 (219) |
a
SPy numbers are based on annotation of the serotype M1 genome sequence.
b
Protein quantities were determined with the PDQuest software by adding the values for the pixels comprising a protein spot.
c
ND, not detected.