TABLE 3.
Migration and abundance of stress-associated proteins isolated from wild-type and rgg mutant strains
| SPy no.a | Gene product | Observed pI/Mr | Predicted pI/Mr | Mean amt (SEM) after growth forb:
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| 4 h
|
24 h
|
48 h
|
|||||||
| Wild type | rgg mutant | Wild type | rgg mutant | Wild type | rgg mutant | ||||
| 0158 | Anion resistance | 5.3/48.5 | 5.3/46.3 | NDc | ND | ND | 478 (243) | ND | 1,338 (125) |
| 0395 | ClpP | 5.7/22.1 | 5.5/21.6 | ND | ND | ND | ND | 411 (102) | 815 (183) |
| 0888 | ClpL | 6.1/75.3 | 5.8/77.3 | ND | 266 (170) | ND | 1,622 (145) | ND | 1,107 (135) |
| 0888 | ClpL | 6.2/74.4 | 5.8/77.3 | ND | 289 (210) | ND | 2,567 (489) | ND | 1,806 (519) |
| 1509 | ClpE | 5.5/82.3 | 5.3/84.7 | ND | 1,494 (153) | ND | 2,783 (650) | ND | ND |
| 1760 | DnaK | 4.5/69.2 | 4.6/64.9 | 8,365 (1,969) | 9,378 (223) | 15,600 (8,845) | 19,695 (1,350) | 10,123 (2,557) | 7,555 (1,951) |
| 2070 | GroEL | 4.7/57.3 | 4.8/57.1 | 16,449 (3,138) | 11,585 (2,186) | 5,975 (2,468) | 12,918 (1,640) | 6,700 (1,408) | 7,196 (421) |
| 2079 | Ahp | 4.6/20.6 | 4.7/20.5 | 2,785 (78) | 10,939 (1,945) | 674 (674) | 3,190 (3,190) | 4,103 (72) | 10,243 (1,526) |
| 2080 | NoxI | 4.8/53.8 | 4.9/54.8 | ND | ND | ND | 751 (751) | ND | 2,795 (2,795) |
| 2216 | HtrA | 5.3/31.0 | 7.6/42.7 | ND | 423 (170) | ND | 651 (27) | ND | 564 (139) |
a
SPy numbers are based on annotation of the serotype M1 genome sequence.
b
Protein quantities were determined with the PDQuest software by adding the values for the pixels comprising a protein spot.
c
ND, not detected.