TABLE 6.
H-NS modulates the production of TBARSa
| Strain | Pi (mM) | Growth phase (h of growth) | Glucose consumed (mM) | Mean TBARS concn, nmol/mg, ± SD (fold increase) |
|---|---|---|---|---|
| ENZ1203 | 5 | Exponential (7) | 3.3 | 0.05 ± 0.01 |
| ENZ1203 | 0.1 | Stationary (24) | 8.1 | 0.41 ± 0.02 (8.2) |
| hns | 5 | Exponential (22) | 2.1 | 0.15 ± 0.02 (3) |
| hns | 0.1 | Stationary (47) | 9.8 | 0.20 ± 0.03 (4) |
a
Strains ENZ1203 and ENZ1667 (ENZ1203 hns::neo) were inoculated (125 μl from overnight cultures in LB medium) into 100 ml of MOPS (5 mM Pi plus 20 mM glucose) or Pi-limiting medium (0.1 mM Pi plus 20 mM glucose) at time zero. Exponentially growing cells in MOPS medium were taken at an OD600 of 0.7 (hns+; time = 7 h; generation time ≈ 52 min) or at an OD600 of 0.4 (hns mutants; time = 22 h; generation time ≈ 3 h). TBARS values are the means of two experiments. The values relative to those in hns+ cells in the exponential growth phase are indicated in parentheses.