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Cartoon summarizing cross-linking studies with the constructs described in the text. Relative placement of native cysteines is shown on a cartoon backbone of Aer. Arrows pointing toward FAD represent an inability of these constructs to bind FAD. The letter x shows the relative positions of R235C and R235E replacements. Cross-linking was not tested in an Aer protein containing a single cysteine at residue 235 (Aer-C193S/C203A/C253A/R235C).
