TABLE 1.

Summary of cross-linking data

Aer construct	Position(s) where cysteine is present	FAD binding	Signaling	Avg % cross-linkinga
Wild type	193, 203, 253	+	+	19 ± 3
C193S/C203A	253	+	+	14 ± 2
C193S/C253A	203	+	+	5 ± 2
C203A/C253A	193	+	+	0
C193S/C203A/C253A		+	+	0
His6x-Aer2-285b	193, 203, 253	+	−	27 ± 1
His6x-Aer2-231b	193, 203	−	−	12 ± 2
R235Cc	193, 203, 235, 253	−	−	Yesd
R235Ec	193, 203, 253	−	−	Yesd

^aCross-linking was quenched at 10 min. Standard deviations were determined from three experiments.

^bFAD binding and signaling data are from reference 6.

^cFAD binding and signaling data are from reference 14a.

^dPercent cross-linking was not quantified.