TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| E. coli | ||
| DH5α λpir | φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR λ-pir | J. J. Mekalanos |
| GM2163 | F−ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam-13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2 | New England Biolabs |
| S17.1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 λ-pir | 42 |
| P. fluorescens | ||
| Pf0-1 | Wild type, Ampr | 6 |
| Pf0-2x | Pf0-1 adnA::Sm/Sp | 38 |
| Pf0-1Δdap | Pf0-1 ΔdapB | This study |
| Pf0-1::iiv2Sm | Pf0-1 with partial deletion of iiv2 | This study |
| Pf0-1::iiv3Sm | Pf0-1 with partial deletion of iiv3 | This study |
| Pf0-1::iiv7Sm | Pf0-1 with partial deletion of iiv7 | This study |
| Plasmids | ||
| pGEM-T Easy | Apr; cloning vector for PCR products | Promega |
| pHRP315 | pMB1 origin; Apr; ΩSm/Sp cassette | 30 |
| pHRP317 | pMB1 origin; Apr; ΩSm/Sp cassette | 30 |
| pSR47s | Kmr; sacB-containing suicide vector (π-requiring R6K replication origin) | 23 |
| pSRΔdap | pSR47s derivative containing ΔdapB | This study |
| pSRiiv2Sm | pSR47s derivative containing iiv2::Sm | This study |
| pSRiiv3Sm | pSR47s derivative containing iiv3::Sm | This study |
| pSRiiv7Sm | pSR47s derivative containing iiv7::Sm | This study |
| pUIC3 | Universal IVET construct; lacZY′, bla, Tcr, R6K origin | 32 |
| pIVETdap | dapB′ cloned in SpeI site of pUIC3 | This study |