TABLE 2.

Distribution of charged residues in the proposed topological model of Cgs

Cgs region	No. of residues	Locationa	qb	q′c
1-418	418	Cyt	≫	5/0 (q = 5)
442-451	10	Per	1/1 (q = 0)
475-818	344	Cyt	≫	2/2 (q = 0) 5/1 (q = 4)
842-846	5	Per	0/0 (q = 0)
870-938	69	Cyt	8/6 (q = +2)
962-967	6	Per	0/1 (q = −1)
991-2831	841	Cyt	≫	4/4 (q = 0)

^aCyt, cytoplasmic; Per, periplasmic.

^b≫, domains contain much more than 65 residues. q is the net charge calculated as follows: K + R/D + E (positively charged residues/negatively charged residues).

^cq′ is the net charge calculated as follows: K + R/D + E (positively charged residues/negatively charged residues), before or after the TMSs, taking up to 20 amino acid residues into account.