TABLE 1.
E. coli strains and plasmids
| Strain or plasmid | Genotype or characteristic | Reference or source |
|---|---|---|
| Strains | ||
| DE112 | rpsL galK2 Δlac74 tolC::mini-Tn10 | 49 |
| DPD1675 | ilvB2101 ara thi Δ(proAB-lac) tolC::mini-Tn10 | 50 |
| DPD1818 | tolC::mini-Tn10 of MG1655 | This work |
| DPD2410 | yhcS::TN〈Kan〉 of DH5αE | This work |
| DPD2433 | yhcS::TN〈Kan〉 of MG1655 | This work |
| DPD2435 | yhcS::TN〈Kan〉 tolC::mini-Tn10 of MG1655 | This work |
| DPD2443 | yhcP::TN〈Kan〉 of DH5αE | This work |
| DPD2444 | yhcP::TN〈Kan〉 of MG1655 | This work |
| MG1655 | F−rph-1 fnr | 14 |
| Plasmids | ||
| pDEW215 | yciG-luxCDABE | 48 |
| pDEW558 | lysU-luxCDABE | This work |
| pDEW655 | yhcRQP-luxCDABE | This work |
| pKK223-3 | Pharmacia | |
| pDEW659 | yhcP in pKK223-3 | This work |
| pTrcHis2TOPO/lacZ | Invitrogen | |
| pDEW668 | yhcRQP in pTrcHis2TOPO | This work |
| pDEW673 | yhcQP in pTrcHis2TOPO | This work |
| pDEW675 | yhcRQ in pTrcHis2TOPO | This work |