TABLE 2.
Bioluminescence response of the yhcRQP-luxCDABE gene fusion to pHBAa
[pHBA], mM | Relative light units
|
|
---|---|---|
yhcS+ | yhcS | |
0 | 0.631 | 0.815 |
1.6 | 6.72 | 1.02 |
3.1 | 10.2 | 1.16 |
6.2 | 16.2 | 1.42 |
12.5 | 30.8 | 1.82 |
25 | 66.6 | 1.59 |
50 | 91.7 | 0.614 |
100 | 0.437 | 0.045 |
E. coli strains MG1655 carrying pDEW655 and DPD2433 carrying pDEW655 were used. Log-phase cultures in LB medium were treated with the indicated concentrations of pHBA for 30 min.