TABLE 3.
Chemical sensitivities of E. coli strains with and without the YhcP efflux pumpa
| Chemical | MICb for:
|
Fold difference | Rank difference | |
|---|---|---|---|---|
| MG1655 (yhcP+) | DPD2444 (yhcP) | |||
| pHBA | 100 | 12.5 | 8 | 4 |
| 6-Hydroxy-2-naphthoic acid | 20 | 2.5 | 8 | 3 |
| 2-Hydroxycinnamate | 20 | 10 | 2 | 2 |
| 1,5-Dihydroxynaphthalene | 0.5 | 0.25 | 2 | 1 |
| 1,6-Dihydroxynaphthalene | 0.5 | 0.25 | 2 | 1 |
| 2,7-Dihydroxynaphthalene | 0.62 | 0.31 | 2 | 1 |
| 2-Naphthoic acid | 20 | 10 | 2 | 1 |
| Cinnamic acid | 20 | 10 | 2 | 1 |
| 1,4-Naphthalenedicarboxyylic acid | 10 | 10 | 1 | 0 |
| 1-Hydroxy-2-naphthoic acid | 0.62 | 0.62 | 1 | 0 |
| 1-Naphthoic acid | 2.5 | 2.5 | 1 | 0 |
| 2,3-Dihydroxybenzoic acid | 10 | 10 | 1 | 0 |
| 2,3-Naphthalenedicarboxylic acid | 80 | 80 | 1 | 0 |
| 2,6-Dimethoxyphenol | 5 | 5 | 1 | 0 |
| 3,4-Dihydroxycinnamate | 0.62 | 0.62 | 1 | 0 |
| 3,5-Dimethoxy-4-hydroxycinnamate | 10 | 10 | 1 | 0 |
| 3-Hydroxy-2-naphthoic acid | 1.2 | 1.2 | 1 | 0 |
| Benzoate | 25 | 25 | 1 | 0 |
| 2-Biphenylcarboxlic acid | 2.5 | 2.5 | 1 | 0 |
| Dimethyl sulfoxide | 20 | 20 | 1 | 0 |
| Methyl paraben | 3.8 | 3.8 | 1 | 0 |
| Salicylate | 100 | 100 | 1 | 0 |
a
Cultures were grown in Vogel-Bonner minimal medium with glucose as a carbon source and tetrazolium violet to aid visualization of growth.
b
Results for dimethyl sulfoxide are in percent, all other results are in millimolar.