FIG. 2.

^aCategory 1, stationary-phase promoters selected based on the transcriptome analysis (this study); category 2, stationary-phase promoters selected based on the proteome analysis (Wada et al., unpublished); category 3, stationary-phase promoters identified previously (15); category C, reference promoters associated with the growth-related genes; category H, promoters associated with the heat shock-induced genes. DFP vector for the assay of in vivo promoter activity. The test promoter fragment upstream from the initiation codon with BglII and EcoT221 sites at the termini can be inserted in one step into pGRP (the second version of the DFP vector) between BglII and EcoT221 sites. The initiation codon for GFP is regenerated after ligation of the EcoT221 site. Major restriction enzyme sites and the distances from the EcoT221 site are shown.