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Pseudoreversion analysis. Approximately 106 mutagenized aer clones were spread in a line beneath the surface of a semisoft tryptone swarm plate containing 100 μg of ampicillin · ml−1 and 20 μM IPTG. Blebs showing an aerotactic ring (+) can be easily differentiated from blebs caused by mutations in loci other than aer, which do not show an aerotactic ring (-). Aerotaxis was observed in BT3340 (aer tsr tar tap trg recA) after incubating the plate for 24 h at 30°C.
