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Relative FAD content in membranes after Aer induction with 1 mM IPTG. Total membrane-bound FAD was measured with and without induction in BT3312 (aer tsr) as described elsewhere (34). A ratio of 1.2 or less indicates the mutant Aer protein did not bind FAD. FAD ratios are shown for WT Aer (pKW1), the Aer missense mutants D237G, G240R, L251P, C253R, and N34D, and the Aer pseudorevertants N34D/C253R and S28G/D237G.
