FIG. 5.

Complementation of Aer-HAMP and proximal signaling domain mutants by amino acid substitutions in the PAS domain S28G (A), A65V (B), A99V (C), or N34D (D). The distal end of the Aer-HAMP domain is placed at residue C253. The areas of the HAMP domain corresponding to AS-1 and AS-2 are indicated by the filled black rectangle and horizontal bars, respectively. The proximal signaling region is indicated with diagonal bars, and the HAMP connector region is indicated with a line. Amino acid substitutions in Aer shown to the right of each bar were suppressed by the amino acid substitution in the PAS domain shown at the top and recovered aerotaxis, while amino acid substitutions shown to the left of the bar were not suppressed.