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Complementarity of the N34D and C253R substitutions in Aer. Experimental conditions were the same as for Fig. 3, and the plate was incubated for 24 h. Upper row: WT Aer (pKW1), plasmid vector (pTrc99A), and Aer-C253R. Bottom row: Aer-N34D and Aer-N34D/C253R.
