TABLE 1.
Strains, plasmids, and PCR primers
| Strain, plasmid, or primer | Relevant properties or primer sequence (5′ to 3′) | Reference |
|---|---|---|
| Strains | ||
| RP437 | Wild type for chemotaxis | 31 |
| BT3312 | Δaer-1 Δtsr-7028 | 34 |
| BT3340 | Δtsr-7021 Δtar-tap-5201 trg::Tn10 aer-2::kan recA::cat | 23a |
| BT3388 | Δaer::erm Δtsr-7021 Δtar-tap-5201 trg::Tn10 | 42 |
| XL1-Red | ΔmutD5 ΔmutS ΔmutT | Stratagene |
| Plasmids | ||
| pTrc99A | ptrc expression vector, ColE1 lacIq | Pharmacia |
| pGH1 | pTrc99A Aer+ [1-506] | 34 |
| pQH16 | pGH1 Aer-ΔHAMP domain, BstBI and SacI sites | 23a |
| pKW1 | pQH16 Aer-WT HAMP+, NheI site | This study |
| pKW75 | pKW1 Aer[M21R] | This study |
| pKW76 | pKW1 Aer[D35R] | This study |
| pKW77 | pKW1 Aer[Q39R] | This study |
| pKW78 | pGH1 Aer[N34R] | This study |
| pKW79 | pGH1 Aer[C253D] | This study |
| Plasmid templates for suppressor analysis | ||
| pKW2 | pKW1 Aer[D237G] | This study |
| pKW3 | pKW1 Aer[G240R] | This study |
| pKW4 | pKW1 Aer[L251P] | This study |
| pKW5 | pKW1 Aer[C253R] | This study |
| pKW6 | pKW1 Aer[V230D] | This study |
| pKW7 | pKW1 Aer[R235E] | This study |
| pKW8 | pKW1 Aer[L239Q] | This study |
| pKW9 | pKW1 Aer[L239R] | This study |
| pKW10 | pKW1 Aer[L241P] | This study |
| pKW11 | pKW1 Aer[Q248R] | This study |
| pKW12 | pKW1 Aer[L249P] | This study |
| pKW13 | pKW1 Aer[D259H] | This study |
| pKW14 | pKW1 Aer[V260A] | This study |
| pKW15 | pKW1 Aer[S265P] | This study |
| pKW74 | pKW1 Aer[N34D] | This study |
| Primers | ||
| BstBIF | GGTTTCGAATGGCAGATTGTG | This study |
| SacIR | GCTGAGCTCATCGGTGCCTTTCGCCAGCGT | This study |
| NheIF | ATACCCCGCTAGCGGACGATACC | This study |
| NheIR | GTATCGTCCGCTAGCGGGGTATT | This study |
| BstBIR | GTCTTCGAAACAGGCGCTTGC | This study |
| M21RF | GATACCACTCTGAGGTCCACTACCGATCTG | This study |
| M21RR | CAGATCGGTAGTGGACCTCAGAGTGGTATC | This study |
| N34RF | GCAAAGCTATATCACTCATGCTCGTGACACTTTTGTGCAGGT | This study |
| N34RR | CTCACCTGCACAAAAGTGTCACGAGCATGAGTGATATAGCTT | This study |
| D35RF | CTCATGCTAATCGCACTTTTGTGCAGGTGAGCG | This study |
| D35RR | CGCTCACCTGCACAAAAGTGCGATTAGCATGAG | This study |
| Q39RF | GACACTTTTGTGCGGGTGAGCGGCTATACC | This study |
| Q39RR | GGTATAGCCGCTCACCCGCACAAAAGTGTC | This study |
| C253DF | GGTAGGGCAACTTGGCCTGATGGACCGTTGGCTAATTAACG | This study |
| C253DR | CATCGTTAATTAGCCAACGGTCCATCAGGCCAAGTTGCCCTA | This study |