FIG. 8.

Analysis of bfeA-lacZ transcription in B. bronchiseptica bfeA mutant BRM25. Wild-type strain B013N and bfeA mutant strain BRM25 were grown in SS medium and assayed for β-galactosidase activity. Strains harbored plasmid pRK40 (plasmid vector control) or pRK44 (bfeA-lacZ). Error bars represent ±1 standard deviation from the means of independent triplicate determinations. Culture conditions: black bars, iron replete; gray bars, iron depleted; open bars, iron depleted and supplemented with enterobactin.