TABLE 1.

Bordetella strains and recombinant plasmids

Strain or plasmid	Description	Reference
Strains
UT25	B. pertussis; wild-type clinical isolate	23
UT25Sml	B. pertussis; spontaneous streptomycin resistant derivative of UT25	8
PM9	B. pertussis UT25Sml; ΔbfeR	This study
B013N	B. bronchiseptica; spontaneous nalidixic acid resistant derivative of swine isolate B013	2
BRM24	B. bronchiseptica B013N; ΔbfeR	This study
BRM25	B. bronchiseptica B013N; bfeA::pSS18	This study
Plasmids
pKP1	pUC19 derivative containing the 4.1-kb bfeR bfeA genetic region from B. pertussis strain 82	5
p3Z100	pGEM3Z with 0.4-kb EcoRI insert fragment containing the bfeA-bfeR intergenic region	This study
p3Z105	pGEM3Z with 0.4-kb EcoRI-BamHI insert fragment containing the bfeA-bfeR intergenic region	This study
p3ZFBS	pGEM3Z containing an E. coli consensus Fur-binding site	63
pSS11	pSS1129 with 1.1-kb EcoRI-HindIII insert containing B. pertussis DNA region with the 594-bp PstI fragment of bfeR deleted	This study
pSS18	pSS1129 with 1.2-kb HindIII-BamHI insert containing internal region of B. bronchiseptica B013N bfeA gene	This study
pBB31	pBBR1MCS with 1.7-kb SalI-StuI insert containing B. pertussis strain 82 bfeR gene from pKP1	This study
pBB36	pBBR1MCS-5 with 1.7-kb SalI-StuI insert containing B. pertussis strain 82 bfeR gene from pKP1	This study
pBB37	pBBR1MCS with 2.9-kb PstI insert containing B. pertussis strain 82 bfeA gene from pKP1	This study
pEG7.2	pEG7 with 3.8-kb BamHI sacBR and 1.4-kb BamHI-HindIII ΔbfeR insert fragments	This study
pRK40	pRK415 with 3.3-kb EcoRI-HindIII trp′-′lacZ insert fragment	64
pRK43	pRK40 with 0.4-kb EcoRI bfeA′-bfeR′ intergenic region from p3Z100; bfeR-lacZ transcriptional fusion	This study
pRK44	pRK40 with 0.4-kb EcoRI-HindIII bfeR′-bfeA′ intergenic region from p3Z105; bfeA-lacZ transcriptional fusion	This study
pMP1	pMP220 with 0.4-kb EcoRI-BamHI bfeR′-bfeA′ intergenic region from p3Z105; bfeA-lacZ transcriptional fusion	This study