TABLE 1.
A. tumefaciens strains and plasmids used in this study
| Strain or plasmida | Relevant characteristics | Antibiotic resistanceb | Reference or source |
|---|---|---|---|
| Strains | |||
| A136 | Nononcogenic derivative of C58; lacks pTi | Rif | 35 |
| A208 | Oncogenic nopaline-type strain; contains pTiT37 | Rif | 31 |
| A281 | Oncogenic agropine-type strain; contains pTiBo542 | Rif | 31 |
| A348 | Oncogenic octopine-type strain; contains pTiA6 | Rif | 14 |
| At221 | Tn3-HoHoI insertion in virE2; A348mx358 | Car, Rif | 32 |
| At333 | UIA143 | Ery | 12 |
| At902 | LBA4404(pUCD3960) | Rif, Spe | 19 |
| At903 | LBA4404(pUCD5533) | Rif, Spe | 19 |
| At906 | At221(pUCD3960) | Car, Spe | 19 |
| At907 | At221(pUCD5533) | Car, Spe | 19 |
| At928 | A208(pBISN1) | Kan, Rif | 19 |
| At931 | A208(pBISN1, pUCD3960) | Car, Kan, Rif, Spe | 19 |
| At932 | A208(pBISN1, pUCD5533) | Car, Kan, Rif, Spe | 19 |
| At986 | A208(pBISN1, pJB31) | Kan, Spe | This study |
| At998 | A136(pJW323) | Kan, Rif | This study |
| At999 | A348(pJW323) | Kan, Rif | This study |
| At1000 | A208(pJW323) | Kan, Rif | This study |
| At1001 | A281(pJW323) | Kan, Rif | This study |
| At1002 | A136(pUCD5533) | Car, Rif, Spe | This study |
| At1003 | A348(pUCD5533) | Car, Rif, Spe | This study |
| At1004 | A208(pUCD5533) | Car, Rif, Spe | This study |
| At1005 | A281(pUCD5533) | Car, Rif, Spe | This study |
| At1006 | A348(pJW323, pUCD5533) | Car, Kan, Rif, Spe | This study |
| At1007 | A348(pJW323, pUCD3960) | Car, Kan, Rif, Spe | This study |
| At1008 | A136(pUCD3960) | Car, Rif, Spe | This study |
| At1009 | A348(pUCD3960) | Car, Rif, Spe | This study |
| At1011 | A281(pUCD3960) | Car, Rif, Spe | This study |
| At1016 | A136(pSa) | Chl, Kan, Rif, Spe, Suf | This study |
| At1017 | A348(pSa) | Chl, Kan, Rif, Spe, Suf | This study |
| At1018 | A208(pSa) | Chl, Kan, Rif, Spe, Suf | This study |
| At1019 | A281(pSa) | Chl, Kan, Rif, Spe, Suf | This study |
| At1020 | A136(pSa::neo) | Chl, Kan, Neo, Rif, Spe, Sul | This study |
| At1021 | A348(pSa::neo) | Chl, Kan, Neo, Rif, Spe, Sul | This study |
| At1022 | A208(pSa::neo) | Chl, Kan, Neo, Rif, Spe, Sul | This study |
| At1023 | A281(pSa::neo) | Chl, Kan, Neo, Rif, Spe, Sul | This study |
| At1055 | A348(pML122, pUCD3960) | Car, Gen, Kan, Rif, Spe | This study |
| At1056 | A348(pML122, pUCD5533) | Car, Gen, Kan, Rif, Spe | This study |
| At1057 | A136(pML122) | Gen, Kan, Rif | This study |
| At1058 | A348(pML122) | Gen, Kan, Rif | This study |
| At1059 | A208(pML122) | Gen, Kan, Rif | This study |
| At1060 | A281(pML122) | Gen, Kan, Rif | This study |
| At1076 | A136(pJB31) | Rif, Spe | This study |
| At1077 | A348(pJB31) | Rif, Spe | This study |
| At1078 | A208(pJB31) | Rif, Spe | This study |
| At1079 | A281(pJB31) | Rif, Spe | This study |
| At1080 | A281::osa | Rif, Spe | This study |
| At1081 | A348::vector | Rif, Spe | This study |
| At1084 | A348::osa | Rif, Spe | This study |
| At1096 | A348ΔvirB | Rif | 1 |
| At1111 | At1096::osa | Rif, Spe | This study |
| At1112 | At1096::vector | Rif, Spe | This study |
| At1113 | A208(pBISN1, pED9) | Kan, Rif, Tet | This study |
| At1114 | A208(pBISN1, pED9, pUCD3960) | Car, Kan, Rif, Spe, Tet | This study |
| At1115 | A208(pBISN1, pED9, pUCD5533) | Car, Kan, Rif, Spe, Tet | This study |
| At1116 | A208(pBISN1, pED9, pJB31) | Kan, Rif, Spe, Tet | This study |
| At1148 | At1081(pML122) | Gen, Kan, Rif, Spe | This study |
| At1149 | At1084(pML122) | Gen, Kan, Rif, Spe | This study |
| At1160 | UIA143(pTiA6) | Ery, Rif | 5 |
| At1181 | UIA143(pTiA6, pE1649) | Ery, Spe, Tet | This study |
| At1182 | UIA143(pTiA6, pE1650) | Ery, Spe, Tet | This study |
| At1312 | At902(pJW323) | Kan, Rif, Spe | This study |
| At1313 | At903(pJW323) | Kan, Rid, Spe | This study |
| At1314 | At906(pJW323) | Car, Kan, Spe | This study |
| At1315 | At907(pJW323) | Car, Kan, Spe | This study |
| At1368 | LBA1251(pTiA6, pML122, pUCD3960) | Car, Gen, Rif, Spe, Tet, | This study |
| At1369 | LBA1251(pTiA6, pML122, pUCD5533) | Car, Gen, Rif, Spe, Tet | This study |
| LBA1251 | C58 chromosome, lacks pTi and pAtC58 | Rif | Paul Hooykaas |
| LBA4404 | Disarmed octopine-type strain; lacks T-DNA | Rif | 28 |
| Plasmids | |||
| pAD1361 | Complete virB operon plus virGN54D | Car, Tet | 10 |
| pBISN1 | T-DNA binary vector; contains a nos-nptII and superpromoter-gusA intron gene | Kan | 26 |
| pJB31 | RSF1010 derivative | Spe | 2 |
| pJW323 | RSF1010 derivative, contains a nos-nptII plant-selectable marker | Kan | 34 |
| pML122 | RSF1010-derived broad-host-range vector | Gen, Tet | 18 |
| pE578 | IncP plasmid carrying the pgl/picA locus as an EcoRI fragment from the A. tumefaciens C58 chromosome | Tet | 30 |
| pE1649 | PstI fragment containing the osa and spectinomycin resistance genes cloned into pE578 | Spe, Tet | This study |
| pE1650 | PstI fragment containing a spectinomycin resistance gene cloned into pE578; vector control for osa construction | Spe, Tet | This study |
| pED9 | virB, virB10, and virB11 genes under the control of the pTiA6 virB promoter | Tet | 34 |
| pPH1JI | IncP plasmid; used for eviction of other IncP plasmids during marker exchange mutagenesis | Gen | 15 |
| pSa | Oncogenic suppressive plasmid | Chl, Kan, Spe, Sul | 23 |
| pSa::neo | Neomycin phosphotransferase gene disruption of the osa gene of pSa | Chl, Kan, Neo, Spe, Sul | 19 |
| pUCD3960 | osa gene under the control of an npt promoter in pUCD105 | Car, Spe | 19 |
| pUCD5533 | Asp718-blunted derivative of pUCD105 | Car, Spe | 19 |
a
Laboratory stock designations: A, E. W. Nester; At, S. B. Gelvin; UCD, C. I. Kado; AD, A. Das.
b
Car, carbenicillin; Chl, chloramphenicol; Ery, erythromycin; Gen, gentamicin; Kan, kanamycin; Neo, neomycin; Rif, rifampicin; Spe, spectinomycin; Sul, sulfonamide; Tet, tetracycline.