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. 2004 Oct 19;2(11):e352. doi: 10.1371/journal.pbio.0020352

Figure 7. The Late-1 Element Negatively Regulates R07B1.9.

Figure 7

(A) A portion of the promoter sequence of R07B1.9 from C. elegans (bottom) aligned with its ortholog from C. briggsae (top). Boxed regions show conserved predicted PHA-4 binding sites and a Late-1 element. The site-directed mutation that disrupts Late-1 (“Mut”) is shown below the respective wild-type sequence from R07B1.9.

(B and C) Confocal images of representative early and late embryos expressing GFP under the control of the wild-type R07B1.9 promoter.

(D and E) Confocal images of representative early and late embryos expressing GFP under the control of the R07B1.9 promoter with a mutation in the Late-1 element. Note the early activation of R07B1.9 when Late-1 is inactivated.

Dashed lines indicate the outline of the developing pharynx.