Figure 1. Domains and Charge Distribution within the Myosin Tail.

(A) The myosin head (1–818) and light chains are shown at the N terminus. In the coiled-coil tail, Ala 1 is red (1,348–1,530), the AD is blue (1,531–1,824), Ala 2 is purple (1,825–1,966), the C-terminal domain is green (1,967–2,116), and the remainder is black (819–1,347). Phospho-threonines (at positions 1,823, 1,833, and 2,029) are indicated by the letter T.

(B and C) Plots of the average charge of each tail domain color coded as in (A). The y-axis is average charge; the x-axis is tail position. Aspartic acid and glutamic acid are assigned –1, lysine and arginine are assigned +1, and all other a.a. are assigned 0. The average charge in (B) was determined with a window size of 14 a.a., and the average charge in (C) was determined with a window size of 28 a.a.. Arrows highlight the 28 a.a. charge repeat in (B) and the 196 a.a. charge repeat in (C).

(D) “Headless” AD-Cterm (3xThr) (1,531–2,116).