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. 2004 Oct 19;2(11):e356. doi: 10.1371/journal.pbio.0020356

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Copyright: © 2004 Hostetter et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The localization of several GFP-RLC-myosin tail fragments during and just after cytokinesis in live Dictyostelium cells are shown. GFP-myosin (row 1) is clearly localized to the early and late cleavage furrow of the dividing cell and to the back end of the resulting daughter cells. By contrast, GFP-RLC-AD-Cterm (3xThr) (row 2) is localized correctly only at the late stages of cytokinesis and in the back end of one daughter cell. GFP-RLC-AD-Cterm (3xAla) (row 3) is localized to the furrow as well as to the back end of a daughter cell, while GFP-RLC-AD-Cterm (3xAsp) (row 4) shows diffuse localization throughout cytokinesis. The scale bar is 10 μm and the time is indicated in min:sec.