Skip to main content
. 2004 Oct 19;2(11):e356. doi: 10.1371/journal.pbio.0020356

Figure 5. Localization of 0.5-μm Beads in Live Dividing Dictyostelium Cells.

Figure 5

(A) Time course of a representative GMO8B Dictyostelium cell (defined in Materials and Methods; contains GFP myosin) during cytokinesis. GFP-myosin fluorescence is shown in green and the beads are shown in red. While GFP-myosin accumulates in the furrow, the beads show no directed motion. The scale bar is 10 μm and time is indicated in min:sec.

(B) Plot of the location of each bead in each of six dividing cells in the six frames imaged during cytokinesis. The axes were defined in each frame to bisect the center of the cell both horizontally and vertically such that the center of the furrow is the origin of the axes. The position of each bead was then plotted relative to these axes. Different beads are represented by different colors. For three of the beads, the trajectory of the bead is shown with arrows. The average location of a cell is outlined on the plot in transparent green.