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(A) Schematic of the strategy for the ssnA gene deletion in the SS2 GD01 strain via double-crossover recombination. The plasmid pSET4sΔssnA is used for the ssnA gene knockout. (B) PCR confirmation of the mutant strain. (C) Growth of the WT GD01, ΔssnA mutant, and its complementation.
