Table 1. Binding data of the recombinant ParmOBP1 and PameOBP2 with different odorants.
| Ligand | PameOBP1 | PameOBP2 | ||
|---|---|---|---|---|
| IC50 (μM) | Ki (μM) | IC50 (μM) | Ki (μM) | |
| Farnesene | 1.14±0.12 | 1.04±0.10 | 0.64±0.08 | 0.52±0.03 |
| Farnesol | 1.01±0.09 | 0.92±0.06 | 0.65±0.07 | 0.53±0.06 |
| 2-Tridecanone | 2.02±0.16 | 1.85±0.17 | 1.52±0.16 | 1.23±0.08 |
| Beta- ionone | 15.77±2.1 | 14.44±1.6 | 15.05±1.1 | 12.17±0.9 |
| Cis-3-hexenyl hexanoate | 24.62±1.9 | 22.54±2.6 | >40 | >40 |
| Tetradecane | >40 | >40 | 1.19±0.09 | 0.96±0.10 |
| Geranylacetone | >40 | >40 | 8.62±0.68 | 6.97±0.72 |
| Trans-nerolidol | >40 | >40 | 15.55±1.30 | 12.57±0.98 |
| Geranyl acetate | >40 | >40 | 39.97±3.16 | 32.57±2.95 |
| Nerolidol | >40 | >40 | 32.90±4.21 | 26.61±2.79 |
Notes: IC50 values of <40 μM were obtained based on the binding curves. Other tested compounds (not listed) that have high Ki values (>40 μM) for all of the OBPs are beta-caryophyllene, alpha-phellandrene, (+/-)-alpha-pinene, 2-pentadecanone, (+)-beta-pinene, ocimene, (-)trans-caryophyllene, ethylbenzene, indole, naphthalene, cumene, tridecane, undecane, dodecane, octadecane, heptadecane, tridecane, benzyl alcohol, 1-hexanol, 2-hexyl-1-decanol, cis-3-hexen-1-ol, cis-2-hexen-1-ol, geraniol, (+/-)-linalool, eucalyptol, alpha-ionol, 2-heptanol, (+)-cedrol, linalool, trans-3-hexen-1, methyl anthranilate-ol, butyl formate, caproyl acetate, pentyl acetate, ethyl propionate, ethyl benzoate, octyl acetate, octyl aldehyde, cis-3-hexenyl acetate, trans-2-hexenyl acetate, phenylacetaldehyde, beta-cyclocitral, (+)-carvone, damascenone, 6-methyl-5-hepten-2-one, 2-heptanone, (±)-camphor, acetophenone, fodecyl aldehyde, decanal, hexyl butyrate, (+)-limonene oxide, (E3)-hexen-1-ol, camphene, (R)-(+)-limonene, phenethyl alcohol, 3-hexanol, Cis-3-hexenol, benzyl acetate, phenethyl acetate, cis-3-hexenyl acetate, ethyl butyrate, trans-2-hexenyl butyrate, phlorizin dehydrate, heptyl acetate, methyl salicylate, butyl acetate, nonyl acetate, isoamyl acetate, cis-3-hexenyl butyrate, benzaldehyde, hexanal, trans-2-hexenal, cis-3-hexenal, 3-hexanone, 2-hexanone, and undecanal.