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. 2017 Jan 10;112(1):133–142. doi: 10.1016/j.bpj.2016.11.3196

Figure 6.

Figure 6

Multiplane diffusion maps of Dendra2-YAP protein in cell nucleus. (A) Dendra2-YAP diffusion maps (similar to Fig. 4C) at different planes in the cell nucleus (z spacing 0.8 μm). See Fig. S9 for mean intensity images. (B) Representative ACF of Dendra2-YAP in the cell nucleus (fit in dashed line). (C) Diffusion coefficient probability distribution (solid black line) of Dendra2-YAP for all seven planes (dashed lines represent ±1 SD). (Gray) Distributions for individual planes. All images were performed on a sCMOS camera with effective pixel size = 160 nm. ACF analysis was performed with 4 × 4 pixel binning. To see this figure in color, go online.