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. 2017 Jan 10;112(1):87–98. doi: 10.1016/j.bpj.2016.11.3197

Figure 1.

Figure 1

Measuring TRPV1 activity using an intramolecular BRET probe (A). Schematic representation of the YFP-TRPV1-Luc intramolecular BRET probe, where YFP was fused to the N-terminal extremity of TRPV1 while Luc was fused to the C-terminal extremity of TRPV1. After activation of TRPV1, the distance (d) and/or the orientation (o) between Luc and YFP were expected to be modified. (B) Kinetic measurement of the effect of 20 μM CAPS on HEK293T cells expressing the YFP-TRPV1-Luc BRET probe. (Star) Time of CAPS injection. Data represent one out of five independent experiments. The time-constant (τ) of the BRET increase induced by CAPS is 2.01 ± 0.29 s, n = 5. To see this figure in color, go online.