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. 2004 Nov;78(21):12075–12081. doi: 10.1128/JVI.78.21.12075-12081.2004

FIG. 1.

FIG. 1.

The construction of two chimeric IRES elements with the reciprocal domain IV/IV(A) of the HCV and CSFV IRES elements. pHCV-IRES-CAT (A) and pCSFV-IRES-CAT (B) represent wild-type HCV and CSFV IRES elements ligated in frame upstream of a CAT gene (18). The shaded AUG in domain IV/IV(A) represents the authentic polyprotein initiation codon. The chimeric IRES element pHCV1-3/CSFV4 (C) contains the HCV IRES domains I to III and the CSFV IRES domain IV(A), and pCSFV1-3/HCV4 (D) contains the CSFV IRES domains I to III and the HCV IRES domain IV. The splice site used to switch the domain IV/IV(A) regions is shown by an arrow, and the respective domain IV regions are outlined.