TABLE 3.
Average copy number of DpnI-resistant oriP replication reporter plasmid (i.e., plasmid 1033) at 4 and 21 days posttransfection in 293 cells expressing EBP2-DBD or EBNA1
| Days posttransfection | Cell line transfecteda (mean copy no. ± SD)
|
|
|---|---|---|
| 293/EBNA1 | 293/EBP2-DBD | |
| 4 | 32 ± 13 | 2 ± 2 |
| 21 | 23 ± 5 | <0.5 |
a
Data represent the means and standard deviations of two experiments. Copy numbers are corrected for transfection efficiency. The average transfection efficiency was 81%, as determined by flow cytometry for a cotransfected GFP expression plasmid.