TABLE 2.
Sensitivity of HIV-1 isolated from heavily drug-experienced individuals against NRTIs and PIs
| Virus | IC50a (μM) (fold change)
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| AZT | ddI | 3TC | ABC | IDV | RTV | SQV | NFV | APV | |
| HIV-1wtb | 0.0060 (1) | 0.20 (1) | 0.22 (1) | 0.13 (1) | 0.017 (1) | 0.055 (1) | 0.0090 (1) | 0.030 (1) | 0.015 (1) |
| HIV-1B | 0.42 (70) | 2.8 (14) | >100 (>455) | 5.0 (22) | >1 (>77) | >1 (18) | 0.25 (28) | >1 (>33) | 0.25 (17) |
| HIV-1C | 0.42 (70) | 1.6 (8) | >100 (>455) | 4.4 (34) | 0.39 (23) | >1 (18) | 0.039 (4) | 0.40 (13) | 0.31 (21) |
| HIV-1G | 0.54 (90) | 1.2 (6) | >100 (>455) | 2.2 (17) | 0.17 (10) | >1 (18) | 0.033 (4) | 0.095 (3) | 0.22 (15) |
| HIV-1ES | 0.75 (125) | 4.3 (>22) | 0.46 (2) | 4.3 (33) | >1 (>77) | >1 (18) | 0.040 (4) | 0.24 (8) | 0.73 (49) |
| HIV-1EV | 0.53 (88) | 4.9 (>25) | >100 (>455) | 5.0 (22) | 0.86 (51) | >1 (18) | 0.50 (56) | 0.60 (20) | 0.55 (37) |
| HIV-1EY | 0.72 (120) | 1.5 (>8) | 0.97 (4) | 1.6 (12) | 0.33 (19) | 0.62 (11) | 0.18 (20) | 0.18 (6) | 0.61 (40) |
a
The IC50 values were determined by using PHA-PBMC exposed to HIV-1s (50 TCID50 dose/105 PBMC) and the inhibition of p24 Gag protein production as an endpoint. All drug sensitivities were determined in triplicate. The values shown are representative of two or three separate experiments. The numbers in parentheses represent the fold changes of the IC50 values against each isolate compared to the IC50 values against HIV-1wt. See Table 1, footnote a, and Materials and Methods for other abbreviations. NFV, nelfinavir.
b
HIV-1wt was a pretherapy clinical HIV-1 strain, HIV-1ERS104pre (36).