FIG. 4.

R increases C-mer protein expression. HeLa cells and 293 cells were infected with AdR or AdLacZ (MOI of 20), and protein extracts were harvested 24 h postinfection. Protein extracts were also obtained from LCLs derived from the same donor with wild-type virus (WT) or virus having BRLF1 deleted (R-KO). HeLa and LCL extracts were immunoprecipitated (IP) and immunoblotted (IB) with a C-mer polyclonal antibody. 293 cell extracts were first pulled down by using WGA-Sepharose beads and then immunoblotted with a C-mer antibody.