TABLE 2.

Detection of defective and standard genomes in virus from plaques formed by C-S8p260 and C-S8p260p3d

Virus and assaya	No. of plaques analyzed	Genomes present in the analyzed plaquesb
		st	Δ417	Δ999 or Δ1017
C-S8p260
First plaque assay	41	−	+	+
	1	+	−	−
Second plaque assay	17	−	+	+
Third plaque assay	3	−	+	+
C-S8p260p3d plaque assay	5	+	−	−

^aThe origin of C-S8p260 and C-S8p260p3d and the procedure for the plaque assays are described in Materials and Methods. Three serial plaque assays were done with various plaques from C-S8p260.

^bRT-PCR amplifications used were numbers 9, 10, 13, and 21, to detect Δ417 and 17, 18, 20, and 21, to detect Δ999 or Δ1017 (Fig. 1A); amplification number 29 shown in Table 1 was used to detect standard (st) RNA.