FIG. 4.
WNV VLPs infect a variety of cell lines with efficiencies similar to those of WNV. Monolayers of Vero, BHK, Huh-7, and C7-10 cells containing 105 to 106 cells were infected in parallel with 10-fold serial dilutions of WNV and VLPs, and infective titers were determined by immunocytochemical staining with WNV-specific MHIAF. WNV titers were 8.4 × 106 PFU/ml on Vero cells, and VLP titers were 1.89 × 108 IU/ml on Vero cells. Titers of the same preparations of WNV and VLPs on all cell lines examined are shown in the graph. The patterns of infection efficiency are very similar, demonstrating an approximately 10-fold drop in titers on BHK and C7/10 cells compared to titers with Vero and Huh-7 cells.