TABLE 1.
WNV IFA-positive mosquito tissues after intrathoracic VLP inoculation
| WNV VLP dose/mosquito | Tissuea
|
||||
|---|---|---|---|---|---|
| Midgut epithelium | Midgut muscle | Midgut trachea | Abdominal fat body | Salivary gland | |
| 0b | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 |
| 3.3 × 102 ± 1.1 × 102c | 0/10 | 0/10 | 2/10 | 2/10 | 1/10 |
| 2.2 × 103 ± 4.2 × 102c | 0/10 | 0/10 | 8/10 | 8/10 | 0/10 |
| 8.2 × 104 ± 1.0 × 104c | 0/10 | 2/10 | 8/10 | 9/10 | 2/10 |
| 5.0 × 105 ± 2.0 × 105c | 0/10 | 3/10 | 10/10 | 10/10 | 4/10 |
a
Tissues were examined by fluorescent microscopy and scored in a blinded fashion. Values are the number of positive tissue samples per the total number of tissues examined.
b
Mock-infected mosquitoes.
c
VLP titers are represented as average titers from three mosquitoes per VLP dose ± the standard deviation, obtained by titration of mosquito homogenates on Vero cells.