FIG. 2.

Delivery of MV N to the cell surface requires FcγR expression. (A) Tonsillar lymphocytes were activated by interaction with CD40-L and infected with MV Edmonston (MOI, 0.5), and cell membrane expression of N and FcγR was analyzed 48 h postinfection. (B) IIA1.6 cells or (C) IIA1.6 cells transfected with human FcγRIIb1 were infected with a recombinant vaccinia virus expressing MV N protein (MOI, 0.01) for 24 h and analyzed by immunocytometry for expression of N on cell membranes. (D to F) Nonpermeabilized human PBLs, (G to I) P815-N cells, or (J to M) P815-M cells were analyzed by confocal microscopy for cell surface expression of N (red) and FcγR (green). (K) M protein expression (blue) is detected after cell permeabilization. (F and I) Yellow color in the merged panels shows the colocalization of N and FcγR.