FIG. 3.

Intracellular localization of MV N. (A and B) Representative examples of ultrathin sections of P815-N cells, analyzed by electron microscopy. Immunogold labeling revealed N in the cytoplasm, plasma membrane, and cytoplasmic vesicles (arrows). v, vesicle; m, cell membrane. Bars, 200 nm. (C) P815-N or P815-M cells or infected (G954; MOI, 0.1) human B lymphocytes or T lymphocytes were permeabilized and analyzed by confocal microscopy for intracellular expression of N (red), FcγR (green), or cathepsin B (blue). Yellow in merged panels, colocalization of N and FcγR; purple, colocalization of N and cathepsin B; white, all three markers.