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. 2004 Nov;78(21):11904–11915. doi: 10.1128/JVI.78.21.11904-11915.2004

FIG. 3.

FIG. 3.

HIV-1 colocalizes with early endosomes. (A to D) Polarized JAR cells were colabeled with purified human anti-HIV-1 followed by an Alexa 488-conjugated goat anti-human antibody and mouse anti-EEA-1 followed by Texas Red-conjugated goat-anti mouse IgG. The x axis corresponds to the cell layers from the apex to the bottom. The left y axis illustrates the number of pixels (HIV-1 in green bars and EEA-1 in red bars) present over the entire depth of the cells 5 min, 15 min, 30 min, or 2 h following exposure to HIV-1. The right y axis refers to the percentage of colocalization of Ada-M versus EEA-1 (yellow triangles) or EEA-1 versus Ada-M (blue squares) over the entire depth of the cells. (E to H) Confocal images of the 3-μm cell layer following a 15-min exposure to HIV-1. (E) Ada-M is shown in green. (F) EEA-1 is depicted in red. (G) Overlay of signals specific for HIV-1 and EEA-1. (H) Binary image showing the regions of colocalization between Ada-M and EEA-1 on this cell layer (yellow dots). Bar, 10 μm.