Skip to main content
. 2004 Nov;78(21):12085–12089. doi: 10.1128/JVI.78.21.12085-12089.2004

FIG. 2.

FIG. 2.

(A) Comparison of neo- and bsd-resistant replicon efficiencies. Huh7 cells were electroporated with 1 μg of indicated neo- or bsd-resistant replicon RNA. Equal numbers of cells (106) were plated and selected in media containing the pertinent drug. Shown are Coomassie-stained plates following 2 to 3 weeks of selection. (B) Schematic representation of sequential replicon transfections. Huh7 cells previously transfected and selected for maintenance of A2199T neo replicons were transfected with 1 μg of S2204I bsd or, as a negative control, the replication-incompetent Con1 S2204I Pol-fs. (C) Following transfection, 106 cells were plated and selected with the indicated drugs. Shown are Coomassie-stained plates following 2 to 3 weeks of selection.