FIG. 3.

c-Myc does not alter the nuclear localization of Zta. (A) NPC-KT cells were transfected with the indicated amounts of expression vectors, and cells were fractionated as described in Materials and Methods. Zta expression was monitored by Western blot analysis. (B) Immunofluorescence assays were performed on NPC-KT cells transfected with either Zta or Zta plus c-Myc expression plasmids. Cells were stained for Zta with a Zta MAb (Argene) and FITC-conjugated goat anti-mouse immunoglobulin (Biosource). (C) Deconvolution images of NPC-KT cells transfected with either Zta or Zta plus c-Myc expression plasmids. Cells were stained for Zta with a Zta MAb and DAPI (DNA). Merge, overlays of FITC and DAPI images; Merge II, overlays of FITC, DAPI, and bright fields. Cells shown are representative examples of multiple cells that were analyzed.