FIG. 3.

Viral production, gPr80^env accumulation, and chaperone protein upregulation in WT- and ts1-infected astrocytes. (A) Virus titers for WT and ts1 infection of C1 cells were determined at 24, 48, and 72 hpi. Virus titers are given as infectious units (IU) per 10³ cells. (B) After mock, WT, or ts1 infection and incubation for 24, 48, or 72 hpi, cell lysates were analyzed for their content of gPr80^env and mature gp70. (C) Under the same conditions, cell lysates were also analyzed for their content of GRP78 and GRP94. All blots were stripped and reimmunoblotted with anti-β-actin antibody as a protein loading control. Intensities of bands corresponding to proteins of interest were normalized to those for β-actin, and differences were determined as shown. All blots shown are representative of three independent experiments.