FIG. 4.

Upregulation of xCT, γ-GCL subunits, and GPx, but not of Cu/Zn and Mn SODs, in ts1-infected astrocytes. (A) After mock, WT, or ts1 infection, C1 cells were incubated for 24, 48, and 72 hpi, and after mock or ts1 infection, PACs were incubated for 72 hpi, at which times cell lysates were analyzed for their content of xCT, γ-GCLC, γ-GCLM, and GPx. (B) After mock or ts1 infection, C1 cells were incubated for 24, 48, and 72 hpi in the presence of absence of BSO, and cell lysates were analyzed for their content of xCT, γ-GCLC, γ-GCLM, and GPx. (C) Uninfected C1 cells were incubated for 24 h in medium containing the indicated concentrations of cystine, and cell lysates were analyzed for their contents of xCT and γ-GCLM. (D) Mock- or ts1-infected C1 cells were incubated for 24, 48, or 72 hpi, and cell lysates were analyzed for their contents of Cu/Zn SOD and Mn SOD. All blots were stripped and reimmunoblotted with anti-β-actin antibody as a protein loading control. Intensities of bands corresponding to proteins of interest were normalized to those for β-actin, and differences were determined as shown. All blots shown are representative of three to four independent experiments.