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Subcellular localization of gB in HSV-infected HEC-1A epithelial cells. HEC-1A cells were infected with wild-type HSV-1 (F) for 6 or 12 h, fixed, permeabilized, and stained as follows: mouse anti-gB MAb 15βB2 and I-59 (red) and simultaneously with sheep anti-TGN46 (green), followed by secondary Alexa 594-conjugated goat anti-mouse IgG and Alexa 488-conjugated donkey anti-sheep IgG.
