FIG. 4 and 5.

Redistribution of gE/gI and TGN46 to cell junctions in the absence of cytoplasmic capsids. HEC-1A cells were left uninfected or infected with wild-type HSV-1 strain KOS or TsProt.A for 6 h (Fig. 4) or 11.5 h (Fig. 5). The cells were fixed with paraformaldehyde, permeabilized, and then stained with rat anti-gE/gI polyclonal antibodies (red) and simultaneously with sheep anti-TGN46 (green) and rabbit anti-β-catenin (blue) antibodies. The cells were washed and stained with secondary antibodies: Alexa 594-conjugated goat anti-rat IgG, Alexa 488-conjugated donkey anti-sheep IgG, and Cy5-conjugated goat anti-rabbit IgG.