Skip to main content
. 2004 Nov;78(21):11823–11832. doi: 10.1128/JVI.78.21.11823-11832.2004

FIG. 1.

FIG. 1.

High-molecular-weight Tax products exist in transfected cells and Tax-producing T lymphocytes. (A) Schematic representation of the Tax and ubiquitin (Ub)-Tax proteins. (B) Detection of Tax products by Western blot in 293T cells transfected with the pSG5 control plasmid (lanes 2 and 5) or plasmids encoding ubiquitin-Tax (lanes 1 and 4) or wild-type (wt) Tax (lanes 3 and 6). Proteins were revealed with patient sera (lanes 1 to 3) or an anti-Tax monoclonal antibody (lanes 4 to 6). (C) Western blot performed on equal amounts of uninfected Jurkat (lane 4) or infected C8166 (lane 5) T cells with patient sera. 293T cells transfected with the ubiquitin-Tax (lane 1), Tax (lane 3), or pSG5 (lane 2) plasmids are also shown.