Total and free β-catenin in EBV-negative and -positive cell lines. (Upper) Ten micrograms of total cell lysates from EBV-negative cells, BJAB, DG75, HL60, and Jurkat, and EBV-positive cells, B95-8, cord blood infected with B95-8 virus (CB-B95-8), P3HR1, and RAJI, were analyzed by Western blotting for total β-catenin expression. (Lower) Free β-catenin was determined by incubation of 200 μg of whole-cell lysate with GST-E-cadherin fusion protein. Bound proteins were then eluted and analyzed by Western blotting for β-catenin.