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β-Catenin and phosphorylated GSK3β levels and localization in LCLs. PBL starting material and LCLs transformed with EBV were analyzed by Western blotting. Nuclear, cytoplasmic, and whole-cell extracts of LCL 5000 and LCL 5077 (25 μg each) and whole-cell extract of PBL 5000 (50 μg) were analyzed for β-catenin, actin, GRP78, P-GSK3β, and total GSK3. Whole-cell extracts were diluted as indicated in serial twofold dilutions.
