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IMYMV Rep binds to pea nuclear PCNA. Lane 1 shows ∼50 ng of GST-PCNA. About 2 μg each of 27-kDa GST (lane 3) and 43-kDa Rep (lane 4) was used to reveal the specificity of the antibodies. Input pea nuclear extract (100 μg), resolved by SDS-10% PAGE and stained with Coomassie blue, is shown in lane 5. NE, nuclear extract; IP, immunoprobe.
